Branson digital sonifier 450 manual

A When you want to Enter the TIME duration value. S Send all report data to the remote terminal. P Print all report data, if a printer is enabled. R Recall a particular preset. H Pause operation of the Digital Sonifier until you send another! H command. MT Enter the Maximum Temperature value.

TS Test the system for two seconds. ON Start the experiment. OFF Stop the experiment. KL Lock out the keypad on the front panel. The keypad is enabled again if you send another! KL command, or if you turn the power off on the Digital Sonifier and then turn it on. SV Save the parameters entered. RP View all the test or experiment parameter values. The fuse is a glass slow-blow type refer to the data tag for the fuse rating.

This connection requires a cable with a 9-pin male connector. Serial ASCII data is transferred through this connection at a user-selectable baud rate of , , , or bps with 8 data bits, 1 stop bit, and no parity. A Digital Sonifier System Features 2. On receipt of your Digital Sonifier system, take the following steps: 1. Inspect the carton for signs of damage. Open the carton and locate the packing list. Carefully unpack the components and check them against the packing list.

Save all packing materials in case the equipment needs to be shipped. Inspect the components for any damage that may have occurred during shipping. Report all shipping damage to your carrier. A Installation and Setup 4. The items inside the bold outline are found in the Digital Sonifier unit.

Some elements shown are optional. The computer interface, software and setup are for the reader to determine. This item is sold separately by Branson. Refer to the Appendix B, Optional Items Parts List, for information about specific parts needed for the optional items.

Microtips Particularly useful for processing small volumes, microtips are available in several designs, tapered and stepped, to meet your application requirements. A Coupling Section may be used with a microtip horn for certain applications. Microtip failure may result. Breakage of glassware may result in the loss of a specimen. Disruptor horns are the base for many microtip applications. Disrupter horns are threaded so that they may also be inserted into a Flow-Thru chamber attachment see page When threaded together, a closed chamber is formed between the Tissue Disruptor and the cavity of the Flow-Thru Attachment.

The Tissue Disruptor may also be used alone. The amplitude at the end of a tapered tip is three and one-half times greater than that of the standard horn. The tapered tip is recommended for difficult applications such as spores, fungi, yeast, muscle, and connective tissue.

Excellent results can be achieved on volumes ranging from 3 to 20 ml in a comparatively short period of time. The stepped microtip is a two-piece unit, consisting of a Coupling Section and a lower doublestep tip. Because the coupling section is attached directly to the converter, the standard disruptor horn must be removed prior to using the stepped microtip.

Recommended for use on extremely small volumes, the stepped microtip can be used to treat volumes ranging from 0. Applications for this tip include red and white blood cells, tissue culture cells, HeLa cells, and the complete range of cells that have low to medium resistance to breakage.

To prevent foaming or aerosoling while processing small quantities with the tapered or stepped microtips, the use of a conical-shaped tube such as a reaction vial or a cut down centrifuge tube is recommended.

The microtip will break if driven at higher amplitudes. Tissue Disruptor Designed for disintegration of difficult tissues, this stainless steel accessory has a specially constructed cell bottom that holds up to six grams of tissue. A water jacket may be provided for cooling. A Cup Horn is attached directly to the converter, and the assembly is mounted upside-down on the lab stand with the Cup Horn at the top.

With chilled water in the Cup Horn, test tubes are suspended in the Cup with the contents of the tubes just below the water level. Ultrasonic energy is then conducted from the surface of the horn, through the water and test tube walls, to the contents of the tube. Some energy loss may occur when applying ultrasonics in this indirect way, and processing can take longer than if the ultrasonic horn were immersed directly in the solution.

There are two types of Cup Horn, a high intensity unit that will accommodate a single test tube, and a larger unit for multiple test tubes. The high intensity Cup Horn has a concave bottom that focuses the ultrasonic energy on the bottom of the tube. The larger unit, with a diameter of two or three inches, allows the immersion of multiple test tubes. The larger Cup Horns have clear plastic walls, which permit easy viewing of the activity in the tubes during processing.

Both types of Cup Horn are designed to allow chilled water to circulate through the cup to prevent heating of the solution as a result of the ultrasonic activity. A The bottom of the test tubes should not be in contact with the surface of the ultrasonic horn. Such contact could cause breakage and loss of sample. A pre-mixed substance is fed through one of the inputs while the horn is ultrasonically activated. Because two inputs are available, two different types of material can be treated simultaneously for mixing or emulsifying.

The processed solution exits at the tip of the horn. The horn can be used as a continuous-flow device to collect the solution in one large vessel. A Digital Sonifier System Component Description This attachment, which is screwed onto the disruptor horn, permits continuous processing of low-viscosity materials at rates of up to 38 liters per hour. Designed primarily for emulsifying, dispersing, and homogenizing, the attachment will disrupt most cells, with the exception of the more difficult species.

The materials being treated can be passed through the attachment more than once to obtain desired results. For cooling, a water jacket with input, output, and overflow connections is provided. Rosett Cell The Rosett Cell, made of borosilicate glass, has a conical shape with three side arms, through which the solution is driven by pressure produced from vibrations from the ultrasonic horn, thereby exposing the substance to ultrasonic energy repeatedly during circulation.

When the Rosett Cell is immersed in a cooling bath, the enlarged glass surface area and circulation through the side arms provide an efficient means of heat exchange. The Rosett Cell is available in three sizes: ml, ml, and ml. Flow-Thru Rosett Cell The Flow-Thru Rosett Cell is equipped with its own water cooling jacket, with intake and output connections for continuous processing and a double chamber for cooling.

Normally, adequate cooling can be achieved by connection to the cold water tap or by using a closed circuit system. Since the double chamber is made of glass, the substance can be easily observed during treatment. The FlowThru Rosett Cell is not suited for difficult cells. A Installation and Setup Soundproof Enclosure Although ultrasound is above the normal range of human hearing, audible sound sometimes occurs when liquids are treated ultrasonically, especially due to cavitation produced by ultrasonic vibration.

The Soundproof Enclosure can be used to reduce this to an acceptable level. It is especially useful when the Sonifier is used for extended periods of time. The Soundproof Enclosure is also useful at minimizing splashing while the experiment is running. Cooling within the enclosure may be required for certain applications. Detail of the enclosure may vary from those depicted below. A Digital Sonifier Assembling the Equipment 4. Some assembly of the ultrasonic horn is required, as described in the following sections.

Secure the clamp on the converter housing. To remove a horn, use spanner wrenches shipped with the system. Never attempt to remove a horn by holding the converter housing in a vise. If necessary, secure the largest portion of the horn in a soft-jawed vise. See Section 4. Excessive silicone grease can diminish performance. The recommended torque is inch-pounds A standard flat tip, recommended for processing liquids, is supplied with tapped horns.

Other tip configurations are available for experimental work on applications where the ultrasonic vibrations are transmitted directly into the solution. The shape of the horn influences the direction in which the ultrasonic vibrations are delivered from the horn. Connecting the Tip to the Horn To attach the tip to the horn, take the following steps: Step Action Clean contacting surfaces of the horn and tip, and remove any foreign matter from the threaded stud and threaded hole.

See Figure 4. The system is fuse-protected with a replaceable glass fuse, 5x20mm, slow-blow type refer to the data tag on the system. This fuse should never blow under normal operating conditions.

The fuse holder is found on the rear of the unit, as part of the IEC power connector. Refer to Section 2. See Appendix B and C for accessory part numbers. It connects to an IEC-type connector on the rear of the unit. The plug end connects to your main voltage receptacle, which should be properly fused depending on your site requirements.

A If your cordset does not match your main power receptacle, verify that you have the correct voltage available. Do not connect the system if the voltage rating of the unit is incorrect for your location, as this can damage the unit. The RS connector pin-out is provided below; the connection only supports three data leads Ground, RxD and TxD and does not support handshaking see Remote Computer or Terminal on page for other Setup information.

The serial connection requires a straightthrough cable with a 9-pin DB9 male connector with the following leads supported; the remote end of the cable should match your Remote Terminal device. The remote terminal connection should not exceed a 50 foot 15 meters cable length using conventional data cabling. The terminal is used as a remote terminal device and connects to the unit as such. Your printer cable must have the male DB connector. Plug the printer cable into the connector and turn the printer on.

The Printer must be Set Up in order to function see page The Omega Temperature Probe that is specified matches properly, and is the only temperature device for use with the Digital Sonifier. Use the signal information provided on the previous page. An example of one possible application is shown in the following diagram.

Note: These must NOT be tied to earth ground. Noise due to ground loops could be injected onto the signals. This will cause improper operation, but will not damage the unit.

To avoid injury or accident, never touch the Ultrasonic Horn while the System is turned on, and do not allow the Horn to come in contact with solid vessels or supports.

Later, you can run another test on the system for your particular experiment described in System Performance Benchmark on page Before testing the Sonifier, always make sure that the horn is not touching anything. The System also performs several self-tests when it is first turned on.

To obtain this result 1 Set up the Digital Sonifier following the instructions in this manual. Prepare the Sonifier to operate, if it was not previously assembled.

Verify that the system passes all its self-tests, observing that there are no error messages on the front panel display. Observe the front panel display. Verifies the ultrasonic output of the system.

You may hear a soft, highpitched sound. The bargraph display will show some output value. The test will run for 2 seconds, then stop. Verification that the Digital Sonifier is operating and is ready to be set up for your experiment. No user-accessible adjustments. System settings are covered in Chapter 6. A Technical Specifications 5. It is 0. System performance will vary when you change setup parameters and if your horn or tips change, and can affect the results of your experiments.

Creating a benchmark of your initial setup and performance can be useful at a later date in identifying a change in performance, and can also help in recreating your exact initial setup. The following steps are used only to record system performance, not your experiment results.

Use the following steps to create and record a benchmark for your exact setup. Set up the Digital Sonifier be ready to run , and prepare your experiment.

Turn the Digital Sonifier system On. Note any special immersion comments depth, how it is supported, etc. If you use a Preset, note the Preset ID. Record the serial numbers and horn information of the Digital Sonifier unit, Horn, Converter, and any special equipment. Special information about the Horns is etched into the horn. If you are using the Printer option, you may wish to print a report of the results and keep it on file with the form.

Touching or holding the horn can result in burns or injury, and contacting solid materials with the vibrating horn or tip can cause breakage. Doing so can cause damage to the microtip assembly. System Modes You can control the way in which ultrasonics are applied to your sample by setting the unit to operate in one of several different modes. You determine the mode in which to operate by specifying parameters for your experiment.

You specify the length of the intervals in which ultrasonics are on and off. For example, you can specify 0. The front panel display shows the proper ultrasonics active displays. This allows you to limit temperature buildup in the sample while continuing ultrasonic treatment until you obtain the process results that you want.

This process is repeated until you manually stop the experiment or until the Time that you specified as a limit has elapsed. While the experiment is running, the current and Maximum Temperatures are displayed and updated every second. Limits You may wish to set a Time or Temperature value as a Limit for some experiments. This will cause the experiment cycle to Stop if either the indicated Time has elapsed or the maximum Temperature was reached.

If a Limit is not desired, the parameter should be set to OFF. Temp setting Pulse Temp. The system willrecalculate while running the process Max. TS for the Test command. These commands are also summarized in the Remote Terminal section found on page The fan starts to run, and the system then enters the Self-Diagnostics state. The front panel display shows several diagnostic displays indicating which step in the self-diagnostics it is in and the front panel LEDs will illuminate as a visible test.

After it successfully completes this system check, it enters the Ready state. The front panel display Rev. The system must be in the Ready state for you to start a new experiment or make changes to any parameters. ON command will Start your setup. If you set Limits, your application will Stop automatically upon reaching those limits. TS command to enter the Test state, causing ultrasonics to be sent to the horn. After two seconds, the system returns to the Ready state. P command. After printing, the system returns to the Ready state.

After the report is sent, the system returns to the Ready state. The experiment or test is stopped. You can also use a remote computer or terminal to enter operation parameters.

If a remote computer or terminal is enabled, you can lock out the keypad on the front panel of the unit if you want to operate the unit only from the remote computer or terminal. There are two additional controls Amplitude Control and Power Switch which work with the front panel controls, but are not actually part of the front panel.

The remote computer or terminal may be used to control the system as well. A Operation 6. The remote device can fully control the Digital Sonifier using the built-in serial interface. Instructions for using the remote computer or terminal and the Remote Terminal commands that you can use to enter parameters from your remote computer or remote terminal are described in Section 6.

It rotates up and down to regulate the Amplitude value from minimum to maximum. The Amplitude value is displayed on the Front Panel display. Amplitude may be adjusted while an experiment is running. An example of the Amplitude numerical display is shown in Figure 6. An example of the bargraph display is shown in Section 6. The soft-touch membrane front panel can be permanently damaged. Select buttons, when their parameters are available, will have their LED lit. An error beep or tone will sound if a key that is unavailable is pressed, or if a parameter that is out-of-range has been keyed in.

The system will not accept out-of-range parameters. Be careful to not touch the Horn when a cycle is active, as it may start without warning, and could cause injury.

Each menu line in the display shows a parameter whose value you can change, if its associated Select key LED is lit. The parameter can have either a numeric value that you enter with the numeric keys, or a choice that you select by toggling between a set of possible selections.

You select a parameter for entry by pressing the Line Select key next to it. Then you key in the desired numeric value using the numeric keys, or the appropriate choice from a sub-menu using the Line Select keys. When you press a Line Select key for a numeric parameter, the least significant digit of the parameter value flashes, and the numeric keys become active.

You also use Clear to delete a parameter value to turn a parameter OFF. When you begin entering a value, the old value is replaced in the display by the new one. Once you have started entering a value, the other Line Select keys and the Page Up and Page Down keys become inactive. The Enter key must be pressed to leave the selected parameter.

You select the desired value by pressing the Line Select key next to it and then pressing Enter to accept the value.

The display then returns to the menu. The System Setup parameters can only be accessed from the membrane keypad, not from the remote terminal, and they are not available when a test or experiment is in progress. On the menu, you will see the default values for each of the System Setup parameters. When you are finished with System Setup, exit the Setup menu by pressing the Setup key again. The Setup key becomes inactive, and the display returns to the Parameter Entry menu.

The Enter key must be pressed before leaving the Setup menu to store any new parameter value. The parameters available at System Setup and their possible values are described in the table below. Detailed steps for each parameter are described in the pages that follow. See page for additional information on Setting the Date. Take this action… Press the Setup key to display the System Setup menu. Its LED lights. To get this result… Setup Use the Up and Down arrow keys, if necessary, to display the line showing the Language parameter.

Press the Line Select key that corresponds to Language. Press the Enter key to store the choice for Language. Enter Press the Setup key to exit the System Setup menu. The Setup LED extinguishes. Setup Rev. To get this result… Setup Use the Up and Down arrow keys, if necessary, to display the line showing the Date parameter.

Press the Line Select key that corresponds to Date. A Digital Sonifier Use the numeric keys to enter the current date, using an American or European date format as appropriate. Press the Enter key to store the value for Date, or press the Clear key to re-enter the date.

To get this result… Setup Use the Up and Down arrow keys, if necessary, to display the line showing the Time of Day parameter. Press the Line Select key that corresponds to Time of Day. To get this result… Setup Use the Up and Down arrow keys, if necessary, to display the line showing the Temperature parameter. Press the Line Select key that corresponds to Temperature.

Press the Enter key to store the value for Temperature units. To get this result… Setup Use the Up and Down arrow keys, if necessary, to display the line showing the Printer parameter.

Press the Line Select key that corresponds to Printer. Press the Enter key to store the value for Printer. A Operation System Setup - Printer Model The Model parameter selects the printer model from several choices, when the Printer parameter is enabled.

To get this result… Setup Use the Up and Down arrow keys, if necessary, to display the line showing the Model parameter. Press the Line Select key that corresponds to Model. Press the Enter key to store the value for Model.

To get this result… Setup Use the Up and Down arrow keys, if necessary, to display the line showing the Reports parameter. Press the Line Select key that corresponds to Reports. Press the Enter key to store the value for Reports. Only the Amplitude value using the Amplitude knob on the side of the chassis may be changed when an experiment is running.

When the System is in the Ready mode e. In the Parameter Entry menu, you can enter parameters for your test or experiment. Entering Parameters from the Keypad When in the Ready mode, you can press any available Line Select key on the front panel to select a parameter for modification. There are a total of two 2 pages of parameters; use the Arrow Up or Arrow Down keys to see more parameter choices on the display.

Use the number keys to key in a parameter value, and press Enter to store the value or accept the choice, or press Clear to clear a stored numeric entry or to disable a choice and then press Enter to store the cleared or disabled parameter value. The System will not accept parameter values that are out of the range of the system. Once the parameters have been changed and stored, the experiment is ready to run with the new values. Up to 20 Presets may be saved, using the Save and Recall functions described in the following sections.

Presets are saved and recalled using a number, 01 to Presets save all parameters for the setup except for the Amplitude parameter. Presets are saved until they are over-written or cleared, and are maintained in memory even if the system is turned off or unplugged. Instructions and commands that you use to enter parameters from the remote terminal are described in Section 6. Reviewing Parameters on the Remote Terminal If you have disabled the front-panel keypad by sending a!

If both the keypad and the remote computer or terminal are active, the parameters displayed on the front panel will be similar to those described in this section, but any values you enter on the keypad will be updated at the remote computer or terminal only when you request them by issuing the Recall Parameters!

RP command. See Section 6. A Digital Sonifier Entering Operation Parameters The Operation parameters that you can set from the keypad are described in the following table.

The number is not available for entry. This parameter is available only if a temperature probe is connected to the system. The temperature units Fahrenheit or Celsius are determined by the setting of the Temperature parameter, selected during System Setup.

The amplitude affects the amount of power applied during the experiment. A Operation Parameter Entry - Time The Time parameter, if used, identifies the time duration of an experiment, in hours, minutes and seconds, during which ultrasonic vibrations are active and are putting energy into your experiment.

The experiment will stop when the Time entry has elapsed. Time may also be used as a Limit. Take this action… To get this result… Use the Up and Down arrow keys, if necessary, to display the line showing the Time parameter.

Press the Line Select key that corresponds to Time. A Digital Sonifier Entering Operation Parameters 7 8 9 Use the numeric keys to enter a value for Time, how long you want the experiment to run. A Operation Parameter Entry - Maximum Temperature The Maximum Temperature parameter, if used, identifies the maximum temperature allowed in the experiment.

Take this action… To get this result… Use the Up and Down arrow keys, if necessary, to display the line showing the Max. Temp parameter. Press the Line Select key that corresponds to Max. Take this action… To get this result… Use the Up and Down arrow keys, if necessary, to display the line showing the Pulse On parameter. Press the Line Select key that corresponds to Pulse On.

As soon as you have pressed Enter to store the Pulse On value, the system moves to the Pulse Off parameter line. Use the numeric keys to enter the Pulse Off time, in tenths of seconds.

You do not need to Clear both parameters. This can cause the system to operate in an On and Off fashion, which can cause the ultrasonics to be suspended for a period of time, and then reactivate to continue a process cycle without warning. Take this action… To get this result… Use the Up and Down arrow keys, if necessary, to display the line showing the Pulse Temp parameter. Press the Line Select key that corresponds to Pulse Temp. You can enter Operation parameters through the remote computer or terminal, but you must use the keypad to enter System Setup parameters.

You can use your terminal or computer to direct the system to start, stop, or pause the experiment, to send a report to the printer or terminal, or to display the current parameters. The system defaults to the front-panel control mode when it is first powered up. All remote terminal commands begin with an exclamation point. To Enter a parameter value using a Remote Computer or Terminal Step Action 1 Type the remote terminal command that corresponds to the parameter whose value you want to change.

The remote computer or terminal displays a data entry field for the parameter. The system checks the value for the proper format and range for the parameter. If the value is invalid, the system displays an error message. List of parameters The system displays all the current parameters on the remote computer or terminal. S Report The system sends a report to the remote computer or terminal. Issuing another pause command from the remote terminal will cause the experiment to resume, if the experiment is currently paused.

Ultrasonics are activated for a two-second test. RP Rev. FasTrak service requested after order placement will be quoted and accepted based on available time remaining before required ship date.

Operates at a frequency of 40kHz for quiet benchtop sonication Energy mode user-determined input Process monitoring allows operators optimum control over samples Increases repeatability and precision up to 20 sample-processing programs Available standard converter for stack operation or with an ergonomic handheld converter for quick sample processing. Read more. Print Need Help? Want a quote quickly? Submit a QuickQuote request. Probe Diameter All 3. Ideal for sterile sample processing, sonicators with a cup horn apply high intensity sound on multiple samples without direct horn contact.

This indirect sonication enables processing of samples in sealed tubes to eliminate cross contamination. Frequency Output. Pulse Mode Operation. Cooling Method. Probe Diameter. Probe Type. Wattage Output. Manufacturer SKU. Microtips : These smaller, high-intensity tips are ideal for processing smaller samples in Eppendorf vials or similar vessels. Cup horns : These specialized horns permit high-intensity sound to be applied to multiple samples without direct horn contact.

Available in 2. Flow-through horns : These horns offer the ability to pass a process liquid through an intense energy field within the horn itself. Dual ported horns permit the emulsification of two dissimilar liquids in controlled, continuous proportions. Continuous flow attachment : This temperature-controlled chamber permits continuous processing of a flowing liquid through a high-intensity ultrasonic field.

Sealed atmosphere treatment chambers no longer available : These specialty horns have been developed to handle noxious or hazardous samples while isolating them from incidental contact or to contain reactants for metric evaluation. Features Advanced energy control mode delivers precise energy input in continuous or pulse modes. True temperature control manages sample temperature within user-specified limits requires separate temperature probe.

Control modes include time, temperature, and energy.